TCF/LEF (luc) HEK293

Background
TCF/LEF Reporter (Luc) – HEK293 cell line is designed for monitoring the activity of Wnt
/ β-catenin signaling pathway. The Wnt pathway controls a large and diverse set of cell
fate decisions in embryonic development, adult organ maintenance and disease. Wnt
proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to
stabilization and nuclear translocation of β-catenin. β-catenin then binds to TCF/LEF
transcription factors in the nucleus, leading to transcription and expression of Wnt responsive
genes.

Description
TCF/LEF Reporter (Luc) – HEK293 cell line contains a firefly luciferase gene under the
control of TCF/LEF responsive elements stably integrated into HEK293 cells, showing
Wnt pathway-responsive. This cell line is validated for the response to the stimulation of
mouse Wnt3a and to the treatment of the inhibitor of Wnt / β-catenin signaling pathway.

Applications
• Monitor Wnt signaling pathway activity.
• Screen activators or inhibitors of Wnt / β-catenin signaling pathway.

Format
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.

References
Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell
127(3):469-480.
Chen B et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in
tissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.