hNF-KB(Luc)-HEK293

Product Description
The NF-kB reporter (Luc)-HEK293 cell line is designed for monitoring nuclear factor
Kappa B (NF-kB) signal transduction pathways. It contains a firefly luciferase gene
driven by four copies of the NF-kB response element located upstream of the minimal
TATA promoter. After activation by pro-inflammatory cytokines or stimulants of
lymphokine receptors, endogenous NF-kB transcription factors bind to the DNA
response elements, inducing transcription of the luciferase reporter gene.

Format
Each vial contains 2 X 10^6 cells in 1 ml of 10% DMSO.

Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection
kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

References
1. Pessara U, Koch N (1990) Tumor necrosis factor alpha regulates expression of the
major histocompatibility complex class II-associated invariant chain by binding of an
NF-kB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.

2. Baeuerle PA (1998) Pro-inflammatory signaling: last pieces in the NF-kB puzzle?
Curr Biol. 8(1):R19-R22.

3. Takada Y, Kobayashi Y, Aggarwal BB (2005) Evodiamine abolishes constitutive and
inducible NF-kB activation by inhibiting IkBα kinase activation, thereby suppressing
NF-kB-regulated antiapoptotic and metastatic gene expression, up-regulating
apoptosis, and inhibiting invasion. J Biol Chem. 280(17):17203-17212.