RatPDE7A Cell Line

Product Description:
Recombinant HEK293 cell line expressing rat PDE7A (phosphodiesterase 7A, accession
number NM_031080).

Format:
Each vial contains 1 X 106 cells in 1 ml of 10% DMSO.

Introduction:
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B)
have been identified. PDE7 is widely expressed in various tissues, with PDE7A found
primarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7B
are detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads to
elevated intracellular level of cAMP.

References:
Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and
development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol.
Biotechnol. 77 (5): 1167-1173

Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol.
533(1-3):110-117