rhBMP-2

Description:Mature form of disulfide-bonded homodimer which is biologically activeSource:CHO cellMW:Predicted MW: monomer = 13 kDa, dimer = 26 kDaSDS-PAGE: monomer = 16 kDa (reducing condition), dimer = 32 kDa (non-reducing condition)(Due to glycosylation, rhBMP-2 migrates as a 16 kDa protein under reducing conditions and as a 32 kDa protein under non-reducing conditions in SDS-PAGE).Bioactivity:Fully active (determined by its ability to induce alkaline phosphatase production in ATDC5 chondrogenic cells); Typical ED50 = 50-100 ng/mLPurity: > 98% by SDS-PAGE and RP-HPLC analysisEndotoxin: < 0.1 EU/?g rhBMP-2 as determined by the LAL methodPreservative:NoneSterility:0.2 ?m membrane-filtered and packaged aseptically.QC tests:SDS-PAGE, MALDI-TOF and RP-HPLCFormulation:Lyophilized from a sterile filtered solution of 10 mM sodium citrate, pH 3.5Reconstitution:Add sterile distilled water to a concentration of no less than 0.1 mg/mL. Further dilutions should be made in appropriate buffers.Storage and stability:Upon receiving, store the product at -20