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Description: Recombinant human
aFGF (acidic Fibroblast Growth Factor)
is a disulfide-linked monomer protein
consisting of 141 amino acids and
migrates as an approximately 16 kDa
protein under reducing and non-reducing
conditions. Native human aFGF, generated
by the proteolytic removal of the signal
peptide and propeptide, has a calculated mass
of 16 kDa.
Source: Optimized DNA sequence
encoding human acidic Fibroblast
Growth Factor mature chain was
expressed in E. coli.
Formulation: Lyophilized from a 0.2 μm
filtered solution containing 2.5% glycine,
0.5% sucrose, 0.01% Tween-80, 5 mM
glutamic acid, pH 4.5.
Reconstitution: Reconstitute at 0.1-1.0
mg/mL in distilled water. This solution
can then be diluted into other buffers. To
maximize product collection from vial
surface, vortex briefly and then spin
down to recollect the liquid.