BP Blocking buffer(10x)

BP Blocking buffer, 25ml. It contains 5% BSA in TBST. There are risks associated with blocking; a poorly selected blocking agent or excessive blocking can displace or obscure the protein of interest.Therefore, the correct choice of a blocking agent can be critical to a successful immunodetection. For example, dry milk cannot be used with biotinylated or concanavalin-labeled antibodies, since milk contains both glycoproteins and biotin. The analysis of phosphorylated proteins with phospho-specific antibodies can be compromised if using crude protein preparation as a blocking agent. These preparations may contain phosphatases, and the phosphorylated proteins on the blot could become dephosphorylated by this enzyme.