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RT master mix contains RNase inhibitor, dNTPs, reaction buffer , oligo(dT)18 ,random hexamers and a stablizer. The M-MLV revers transcriptase is in a separate vial. This kit is used for first strand cDNA synthesis from RNA. All of the key components are premixed at an optimal concentration. You just simply add your target RNA, M-MLV revers transcriptase and water to start your reaction.