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Red taq polymerase is a formulation of our regular DNA polymerase, which contains a non-toxic and non-hazardous red dye. The red dye provides easy and quick identification of reactions to which the enzyme has been added, and facilitates the confirmation of complete mixing. When the reaction is complete, a sample of the reaction mix can be loaded directly onto the Agarose gel without the need for loading buffer, since the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates towards the positive electrode, thereby providing a means to monitor the progress of the electrophoresis.
The presence of the dye has no effect on routine enzymatic manipulations, although rare exceptions may occur. In order to produce a reaction of sufficient density to allow for the direct loading of a sample onto a gel, we recommend using a minimum of 1.5u per 50µl reaction
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8540 Production Ave. Suit A,
San Diego 92121 USA
Phone: (858) 689-6988
Fax: (858) 225-0288