Endotoxin Removal Maxi Kit

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Product Code: ER-003

Price: $220.00

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Endotoxin Removal Maxi Kit

1. Add 20ml of maxi plasmid into 20-mL Endotoxin removal Buffer in a 50-mL conical tube

2. Mix by inverting the tube a few times and put on ice for about 5-10 minutes until the solution is clear without turbidity;

3. Mix well again by inverting the tube a few times;

4. Incubate the tube at 37 ?C water bath for about 5-10 minutes and the solution shall be turbid or separated;

5. Centrifuge at 3,000 rpm in a low-speed centrifuge at room temperature for 5 minutes with the brake off;

6. Carefully transfer the upper clear layer solution to another 50-mL conical tube;

7. Precipitate plasmid DNA with 0.1 volume of 3 M NaAc (pH 5.4) and 2 volume of 100% ethanol.