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25x TAE solution, Biotechnology Grade, 1LTris-Acetate-EDTA - 25X Liquid Concentrate. Composition of 1X TAE Buffer: 0.04 M Tris-Acetate, 0.001 M EDTA, Final pH 8.0. Useful for DNA Recovery and in-gel manipulations. Better resolution of large DNA fragments. Useful for > 12kb DNA Separations. Low buffering capacity. Buffer Re-circulation may be required. Low ionic strength. 1.6L can prepare 40L of 1X TAE solution